Select Currency
Translate this page


Format: MS WORD  |  Chapter: 1-5  |  Pages: 107  |  1171 Users found this project useful  |  Price NGN3,000





The extracts of the leaves of H.spicigera were screened for the presence of secondary metabolites: alkaloids, glycosides, flavonoids, carbohydrates, tannins, sterols, terpenoids and resins. The results of the phytochemical screening showed all the extracts with the exception of hexane extract to contain the secondary metabolites analysed in high and moderate amounts. Antimicrobial activities of the crude extracts of H.spicigera against a broad spectrum of micro organisms namely: Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Corynebacterium ulcerans, Salmonella typii, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Neisseria gonorrheae and Candida albicans were carried out. All the extracts showed bactericidal activity against the entire antibiotic resistant micro organisms tested with some degree of variations against standard drug, penicillin. The methanolic extract of H.spicigera exhibited significant bactericidal activity at low concentration of 2.5mg/ml while S.aureus, N.gonnorhea and C.albicans were resistant at MBC of 5.0mg/ml. The insecticidal properties of H.spicigera leaf extracts (hexane, ethyl acetate and methanol) tested against Callosobruchus maculatus on cowpea was carried out. Azadirachtin was used as  standard check along with the extracts tested. Ethyl acetate extract showed the highest percent mortality of 98% each at 72hrs, while 6% and 10% were recorded for the emergence of the C.maculatus. Ethyl acetate extract and azadirachtin standard check showed ovi-position deterrent at 4.16% and 2.78% respectively. Hexane extract showed the least percent (2%) seed damage while the control gave the highest percent (88%) of seed damage at 16 weeks. Hyptis spicigera seed oil was characterized using GC-MS and UV-VIS spectrometry for its fatty acid profile, tocopherol and physicochemical properties. The oil content was 21% while unsaturated fatty acids were linoleic acid (71.85 %) and palmitic acid (16.06%) as predominant fatty acids. Tocopherol content was 186.15mg/ml while Vitamin A was absent. The study showed potentials of Hyptis spicigera seed oil to have high oxidative stability which could be suitable for food and beverage as well as other industrial applications while the tocopherol content could improve human health. Purification of the ethyl acetate fraction by High Performance Liquid Chromatography (HPLC) yielded 3-buten-2-enol on the basis of GC-MS. The best separation using the analytical High Performance Liquid Chromatography (HPLC) was achieved at 0.8ml/min. The hydrodistillation of the fresh leaves of H.spicigera gave a colourless volatile oil with yield of 0.65%.The volatile oil gave forty compounds on the basis of GC-MS with low composition of cineole (4.11%) and caryophyllene (2.61%) while α-pinene (12.16%) β-pinene (9.47%) and α-phellandrene (10.19%) were predominant compounds. Crude ethyl acetate extract of H.spicigera leaves was fractionated through solvent separation followed by thin layer and column chromatography. Two relatively pure constituents were obtained and characterized through spectral studies. The structure elucidation of the constituents was established as ursolic acid and its derivative (3β-hydroxy-urs-12-en-28-oic acid) and 3β-hydroxy-20isopropyl-urs-12-an-28-oic using spectroscopic techniques including 1D and 2D NMR such as 1HNMR, 13CNMR, DEPT, HMQC, HMBC, COSY, NOESY as well as MS, and IR. Toxicity studies on the dichloromethane and methanolic extracts of Hyptis spicigera were carried out on mice intraperitoneally. The LD50 was calculated using the method of Karber (LD50, 2534mg/kg) which showed the plant to be moderately toxic based on the WHO toxicity rating. The biological activity of one of the new compounds (ursolic acid) was determined against a trypanosome (T.brucei brucei).The compound was evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and was found to possess antitrypanosoma activity in vitro in a dose dependent pattern at 0.4µg/ml in 3 minutes.


Not What You Are Looking For?

For QUICK Help Call Us Now!

+234 813 2926 373

+234 701 5050 881

Here's what our amazing customers are saying

Emmanuel Essential
Kogi state University
I actually took the risk,you know first time stuff But i was suprised i received as requested. I love you guys 🌟 🌟 🌟 🌟
Very Good
Azeez Abiodun
Moshood Abiola polytechnic
I actually googled and saw about iproject master, copied the number and contacted them through WhatsApp to ask for the availability of the material and to my luck they have it. So there was a delay with the project due to the covid19 pandemic. I was really scared before making the payment cause I’ve been scammed twice, they attended so well to me and that made me trust the process and made the payment and provided them with proof, I got my material in less than 10minutes
Very Good
Gbadamosi Solomon Oluwabunmi
Swift delivery within 9 minutes of payment. Thank you project master
Ajayi Crowther University, Oyo
I was scared at first when I saw your website but I decided to risk my last 3k and surprisingly I got my complete project in my email box instantly. This is so nice!!!
Ahmadu Bello University
I wish I knew you guys when I wrote my first degree project, it took so much time and effort then. Now, with just a click of a button, I got my complete project in less than 15 minutes. You guys are too amazing!
Temitayo Ayodele
Obafemi Awolowo University
My friend told me about iprojectmaster website, I doubted her until I saw her download her full project instantly, I tried mine too and got it instantly, right now, am telling everyone in my school about, no one has to suffer any more writing their project. Thank you for making life easy for me and my fellow students... Keep up the good work
Very Good
Stancy M
Abia State University, Uturu
I did not see my project topic on your website so I decided to call your customer care number, the attention I got was epic! I got help from the beginning to the end of my project in just 3 days, they even taught me how to defend my project and I got a 'B' at the end. Thank you so much iprojectmaster, infact, I owe my graduating well today to you guys...
Ibrahim Muhammad Muhammad
Usmanu danfodiyo university, sokoto
It's a site that give researcher student's to gain access work,easier,affordable and understandable. I appreciate the iproject master teams for making my project work fast and available .I will surely,recommend this site to my friends.thanks a lot..!
Dau Mohammed Kabiru
Kaduna State College of Education Gidan Waya
This is my first time..Your service is superb. But because I was pressed for time, I became jittery when I did not receive feedbackd. I will do more business with you and I will recommend you to my friends. Thank you.
Very Good
Joseph M. Yohanna
Thanks a lot, am really grateful and will surely tell my friends about your website.


  • Select 3 Project Topics of your choice from your Department.
  • Submit the 3 topics to your Supervisor for Approval.
  • Call Our Instant Help Desk on 0813-292-6373 and Get Your Complete Project Material Instantly.
  • All project materials on this website are well researched by professionals with high level of professionalism.


How do i choose a Project Topic?

Look for your department and select any topic of your choice

I have a fresh topic that is not on your website. How do I go about it?

How fast can i get a complete project from your website?

Within 15 minutes if the exact project topic is on our website

Is it a complete research project or just materials?

It is a Complete Research Project i.e Chapters 1-5, Abstract, Table of Contents, Full References, Questionnaires / Secondary Data

One of your topics suites my project, but the case study is different. What do i do?

Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

How do i get my complete project?

Your Complete Project Material will be sent to your Email Address in Ms Word document format

Can I get my Complete Project through WhatsApp?

Yes! We can send your Complete Research Project to your WhatsApp Number

What if my Project Supervisor made some changes to a topic i picked from your website?

Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

Do you assist students with Assignment and Project Proposal?

Yes! Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

What if i do not have any project topic idea at all?

Smiles! We've Got You Covered. Chat with us on WhatsApp Now to Get Instant Help: (+234) 813-292-6373

How can i trust this site?

We are well aware of fraudulent activities that have been happening on the internet. It is regrettable, but hopefully declining. However, we wish to reinstate to our esteemed clients that we are genuine and duly registered with the Corporate Affairs Commission as "PRIMEDGE TECHNOLOGY". This site runs on Secure Sockets Layer (SSL), therefore all transactions on this site are HIGHLY secure and safe!