ABSTRACT
Diabetes is a chronic disease characterized by impaired glucose absorption and intestinal epithelial cell function. Cisampelous owanrensisis benzene fraction has been traditionally used to treat diabetes, but its effects on glucose absorption and intestinal epithelial cell function are not well understood. To investigate the effects of Cisampelous owanrensisis benzene fraction on glucose absorption and intestinal epithelial cell function in Wistar rats with alloxan-induced diabetes. Thirty Wistar rats were divided into three groups: control, diabetes, and diabetes treated with Cisampelous owanrensisis benzene fraction. Glucose absorption and intestinal epithelial cell function were assessed using standard methods. Cisampelous owanrensisis benzene fraction significantly enhanced glucose absorption and intestinal epithelial cell function in diabetic rats compared to the control group.
CHAPTER ONE
INTRODUCTION
1.1 Background of the Study
Diabetes is a chronic disease characterized by high blood sugar levels, and its prevalence is increasing globally (1). According to the World Health Organization (WHO), the number of people with diabetes worldwide has risen from 108 million in 1980 to 422 million in 2014, and this trend is expected to continue, with predictions suggesting that the global diabetes burden will reach 642 million by 2040 (2). The search for alternative treatments has led to the investigation of medicinal plants, such as Cisampelous owanrensisis (3).
Cisampelous owanrensisis is a plant commonly found in tropical regions of Africa and Asia, and has been used in traditional medicine for centuries to treat various ailments, including diabetes (4). The plant contains a variety of bioactive compounds, including alkaloids, glycosides, and phenolic acids, which have been shown to have potential antidiabetic effects (5). The benzene fraction of Cisampelous owanrensisis has been reported to have hypoglycemic activity in animal models of diabetes (6), but its effect on glucose absorption across the intestinal epithelium is not well understood.
Glucose absorption is a critical process that occurs in the small intestine, where dietary glucose is absorbed into the bloodstream (7). In diabetes, impaired glucose absorption can contribute to hyperglycemia and worsen disease severity (8). The intestinal epithelium plays a crucial role in glucose absorption, and alterations in its structure and function can
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