Select Currency
Translate this page


Format: MS WORD  |  Chapter: 1-5  |  Pages: 75  |  995 Users found this project useful  |  Price NGN3,000





Extraction tank, beakers, measuring cylinder, water bath, tests tubes rack, test tubes, sample pipette, calibration pipette, Bunsen burner, matchet, diesel bunder, separating funnels, spatula, cork borer, refrigerator, meter rule, pencil distiller, eccentric oven, incubator, autoclave, clamp stand, inoculating hoop, spirit lamp, masking tape. 
3.1.1   Disposables: Whitman Filter paper, petridishes, face mask, cotton wool, Aluminum foil, filter paper, masking tape, pen, permanent marker, hand gloves, hand towel, injection bottles, stock bottles.
3.1.2   Reagents
1% Aqueous hydrochloric acid 
10% Fenic chloride 
Acetic anhydride 
Dragendorff reagent 
Concentrated sulphuric acid 
Sodium bicarbonate
Concentrated hydrochloride acide
Bromine water
Magnesium metal 
Ammonium hydroxide solution 
Fehlin chloride A and B
Benzene solution 
Chloroform solution
Methanol (redistilled)
Glacial acetic acid 
3.1.3   Solvents:
Sterile distilled water
3.1.4   Media – Nutrient broth 
3.1.5   Organisms:                                      Codes
Staplylococcus aurous              LIO
Escherichia coli                         LIO
LIO – locally isolated organism

3.1.6 Drugs
40 grams of Mangifera indica extract 
40 grams of Carica papaya extract 
1 gram of methanol, ethylaceta, chloroform and N-hexane fractions of Maifera indica and Carica papaya respectively.
Genterincin injection (80mg/2ml) (control).
3.2       Methods:
3.2.1   Collection of Mangifera indica (mango) and Carica papaya
The fresh leaves of mango and pawpaw were collected in February, 2014 at Uyo, in Uyo Local Government Area of Ikwa Ibom State of Nigeria. Identification Number for Mangifera indica: UUH, No: 3C, Carica papaya: UUH, No: 18A. 
3.2.2   Processing of the leaves of Mangifera indica and Carica papaya
The leaves were reduced to smaller sizes using knife and then dried under the sun several days separately. These leaves were further comminuted. The fragments were then stored in a polythene bags free of moisture until required.
3.2.3   Extractions 
600g of the comminuted leaves of Mangifera indica and Carica papaya respectively were macerated separately in methanol (redistilled) 1.5L respectively for 72 hours with intermittent shaking, all in separate tank extract was filtered using cotton wool and the filtrate further filtered using whatma no.1 filter paper to have the methanolic filtrate respectively. The extract were then concentrated to a constant weight. The concentrated extracts were weight separately and stored at 4oC in well closed beakers for further analysis.      
3.2.4   Phytochemical screening:
Phytochemical screening for secondary metabolites in the plant were performed using standard procedures as described by (Sofowora 1993, Trease and Evans, 2006) and Harborne 1998 as follows: Test for alkaloids: about 0.5g each of the leave extract was boiled in 5ml of 1% aqueous hydrochloric acid over a steam bath; the clear filtrate was treated with few drops of dragendoff’s reagents. Turbidity or a pink or red precipitate was taken as preliminary evidence for the presence of alkaloids in the extract (Harborne, 1973). Test for tannins: about 0.5g of each leaves extract was strived with 5ml of distilled water, 5% ferric chloride was added to the filtrates separately. A blue black, green or blue green precipitate was taken as evidence for the presence of tannins (Trease and Evans, 1989).

Test for anthraquinones: About 0.5g of each of the leaves extract was boiled with 5ml of 10% sulfuric acid and filtered while hot. The filtrates were each shaken with 2.5ml benzene, the benzene layers were separately and half it own volume of 10% ammonia solution added

A pink, red or violet coloration in the ammonia phase (lower phrase) indicated the presence of anthraquinone derivative in the extract (Trease et al, 1989). Test for flavonoids: Fe pieces of magnesium metal was added to 5ml of the leave extract plus conc Hcl. The precipitated was taken as evidence of preliminary presence of flavonoids (Trease et al, 1989). Test for saponins: About 0.5g of each plant leaves was shaken with water in a test tube frothing which persisted on warning was taken as a preliminary evidence for the presence of saponins (Wall et al, 1952).
About 0.5g of each extract was added to about 5ml mixture of 5% sodium bicarbonate and Fehling’s solution and boiled. Presence of the brown precipitated was taken as evidence for the presence of saponin.  Test for cardiac glycosides: Lieberman’s test: 0.5g of plant leaves extracts were dissolved in 2ml of acetic anhydride and cooled well in ice. Sulphuric acid was then careful added. A color change from violet to blue green indicated the presence of a steroidal nucleus Shoppee, 1964).
Keller –Killiani test: About 0.5g leaves extract was dissolved in 2ml of glacial acetic acid containing one drop of ferric chloride solution. This was then underplayed with 1ml concentrated sulfuric acid. A brown ring at interface indicated the presence of deoxy sugar of cardenolide. Test for terpenoids: A little of each portion was dissolved in ethanol. To it 1ml of acetic anhydride was added to each followed by the addition of conc. H2SO4,  a change in colour from pink to violet showed the presence of terpenoids (Sofowora, 1993).

3.2.5    Partitioning fro both Mangifera indica and Carica papaya
About 40g of Mangifera indica and Carica papaya crude extract was dissolved separately in 300ml of methanol and 100ml of water was then added to the crude extract. The methanolic aqueous extract was them introduced into the separating funnel in the clamp; the following solvents were added separately in decreasing order of polarity; ethyl acetate, chloroform, and n-hexane to have the different fractions. The solvent portions were carefully collected into a beaker and concentrated to dryness to yield the various fraction extracts. 
The procedures were repeated and each extract fractions stored in clean and well closed beaker at 4oc in a refrigerator for further evaluation.  


Not What You Are Looking For?

For Quick Help Chat with Us Now!

+234 814 010 7220

+233 541 351 187

Here's what our amazing customers are saying

Emmanuel Essential
Kogi state University
I actually took the risk,you know first time stuff But i was suprised i received as requested. I love you guys 🌟 🌟 🌟 🌟
Very Good
Azeez Abiodun
Moshood Abiola polytechnic
I actually googled and saw about iproject master, copied the number and contacted them through WhatsApp to ask for the availability of the material and to my luck they have it. So there was a delay with the project due to the covid19 pandemic. I was really scared before making the payment cause I’ve been scammed twice, they attended so well to me and that made me trust the process and made the payment and provided them with proof, I got my material in less than 10minutes
Very Good
Gbadamosi Solomon Oluwabunmi
Swift delivery within 9 minutes of payment. Thank you project master
Ajayi Crowther University, Oyo
I was scared at first when I saw your website but I decided to risk my last 3k and surprisingly I got my complete project in my email box instantly. This is so nice!!!
Ahmadu Bello University
I wish I knew you guys when I wrote my first degree project, it took so much time and effort then. Now, with just a click of a button, I got my complete project in less than 15 minutes. You guys are too amazing!
Temitayo Ayodele
Obafemi Awolowo University
My friend told me about iprojectmaster website, I doubted her until I saw her download her full project instantly, I tried mine too and got it instantly, right now, am telling everyone in my school about, no one has to suffer any more writing their project. Thank you for making life easy for me and my fellow students... Keep up the good work
Very Good
Stancy M
Abia State University, Uturu
I did not see my project topic on your website so I decided to call your customer care number, the attention I got was epic! I got help from the beginning to the end of my project in just 3 days, they even taught me how to defend my project and I got a 'B' at the end. Thank you so much iprojectmaster, infact, I owe my graduating well today to you guys...
Ibrahim Muhammad Muhammad
Usmanu danfodiyo university, sokoto
It's a site that give researcher student's to gain access work,easier,affordable and understandable. I appreciate the iproject master teams for making my project work fast and available .I will surely,recommend this site to my friends.thanks a lot..!
Dau Mohammed Kabiru
Kaduna State College of Education Gidan Waya
This is my first time..Your service is superb. But because I was pressed for time, I became jittery when I did not receive feedbackd. I will do more business with you and I will recommend you to my friends. Thank you.
Very Good
Joseph M. Yohanna
Thanks a lot, am really grateful and will surely tell my friends about your website.


  • Select 3 Project Topics of your choice from your Department.
  • Submit the 3 topics to your Supervisor for Approval.
  • Call Our Instant Help Desk on +234 814 010 7220 and Get Your Complete Project Material Instantly.
  • All project materials on this website are well researched by professionals with high level of professionalism.


How do i choose a Project Topic?

Look for your department and select any topic of your choice

I have a fresh topic that is not on your website. How do I go about it?

How fast can i get a complete project from your website?

Within 15 minutes if the exact project topic is on our website

Is it a complete research project or just materials?

It is a Complete Research Project i.e Chapters 1-5, Abstract, Table of Contents, Full References, Questionnaires / Secondary Data

One of your topics suites my project, but the case study is different. What do i do?

Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

How do i get my complete project?

Your Complete Project Material will be sent to your Email Address in Ms Word document format

Can I get my Complete Project through WhatsApp?

Yes! We can send your Complete Research Project to your WhatsApp Number

What if my Project Supervisor made some changes to a topic i picked from your website?

Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

Do you assist students with Assignment and Project Proposal?

Yes! Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

What if i do not have any project topic idea at all?

Smiles! We've Got You Covered. Chat with us on WhatsApp Now to Get Instant Help: +234 814 010 7220

How can i trust this site?

We are well aware of fraudulent activities that have been happening on the internet. It is regrettable, but hopefully declining. However, we wish to reinstate to our esteemed clients that we are genuine and duly registered with the Corporate Affairs Commission as "PRIMEDGE TECHNOLOGY". This site runs on Secure Sockets Layer (SSL), therefore all transactions on this site are HIGHLY secure and safe!