Select Currency
Translate this page

ANTIBACTERIAL EFFECT OF NEEM TREE (AZADIRACHTAINDICA) LEAF EXRACT ON STAPHYLOCOCCUS AUREUS

Format: MS WORD  |  Chapter: 1-5  |  Pages: 75  |  356 Users found this project useful  |  Price NGN3,000

GET THE COMPLETE PROJECT

ANTIBACTERIAL EFFECT OF NEEM TREE (AZADIRACHTAINDICA) LEAF EXTRACT ON STAPHYLOCOCCUS AUREUS

 

Abstact

The main focus of this study was to determine the antibacterial effect of Neem tree (Azadirachtaindica) leaf extract on Staphylococcus aureus. The Neem tree leaf extract was tested in terms of the different levels of concentration with (a) 25% concentration, (b) 50% concentration, (c) 75% concentration, (d) 100% concentration on Staphylococcus aureus. It specifically compared the significant differences of the different levels of concentration ofNeem tree leaf extract, and also compared to the positive control. The plant materials were collected and brought to the West Visayas State University Biological Science Laboratory where the experimentation was conducted. Completely Randomized Design (CRD) was the research design used in the study. The interpretation of the results was based on the Claustro et.al zone of inhibition scale. The statistical analysis (One- way ANOVA) was not employed due to the reason that the zone of inhibition is zero. Results of the study revealed that there was no significant differences on the effect of the different levels of Neem tree (Azadirachtaindica) leaf extract on Staphylococcus aureus.

 

Chapter One

Introduction of the Study

1.1 Background to the Study

Philippines is a country that has plenty of resources and biodiversity. Plants in different species just sprout and grow anywhere.Vegetations remain to be the most important part of human existence. Medicinal substances derived from different plants around us have become the most sought sources of medicines. However, a considerable number of people in our generation still do not recognize the value and significance of medicinal plants.  Many people continue to rely on synthetic drugs for treatment of common illnesses despite their being expensive. Basically, the researchers had initiated a study that may provide appropriate answers to some common health problems experienced by human beings. Nowadays, skin problems also proliferate fast and are equally disturbing just like any other diseasesaffecting our communities.  This is the primary reason why researchers conceptualized this study to determine the anti-bacterial components of Neem (Azadirachtaindica) leaves extract and its possible effects against Staphylococcus aureus, the bacteria that concerns most of the common skin problems.

Neem is a tropical tree that has been declared non-toxic to humans. Previous studies revealed that it contains anti-bacterial properties and high-content of anti-oxidant called polyphenols. Neem tree extracts has an endodontic anti-microbial agent, an alternative to frequently employed Sodium Hypochlorite and Chlorhexide; both to inhibit the growth of endodontic pathogens. Sodium Hypochlorite is now used in endodontic during root canal treatments. It is the medicament of choice due to its efficacy against pathogenic organisms and pulp digestion. Chlorexide is a chemical antiseptic. It is effective on Gram-positive and Gram-negative bacteria. Staphylococcus aureus is facultative anaerobic Gram-positve coccus, and is the common cause of staph infections. It is frequently part of skin flora found in the nose and the skin. About 20% of human population are long –term carriers of Staphylococcus aureus can cause a range of illness from skin infection, such as pimples, impetigo, cellulitis follicutis, to life threatening diseases such as pneumonia, meningitis, osteomyelitis, bacteremia and sepsis. It is still one of the five most common causes of nosocomial infections, often causing post-surgical wounds infection.

This study mainly aimed at finding out the antibacterial effect of Neem (Azadirachtaindica) tree leaf- extract on Staphylococcus aureus. Further, it also aimed to find out whether Neem tree leaf extract has significant effect in terms of different levels of concentrations, namely: (a)25% concentration  (b)50% concentration (c)75% concentration (d)100% concentration on inhibition Staphylococcus aureus. Due to the high cost of medicines, unpredictable weather conditions that brought  certain diseases specifically skin diseases, the continuous uplifting of herbal medicines and abundance supply of neem tree were the reason why the researcher conducted this study.

1.2 Statement of the Problem

This study aimed to determine the antibacterial effect of Neem (Azadirachtaindica) leaves extract against Staphylococcus aureus.

It specifically: Determine the significant difference among the different levels of concentration; (a) 25%, (2) 50%, (3) 75%, (d) 100% of Neem tree leaves extract on Staphylococcus aureus as indicated by its zone of inhibition. Determine what sample treatment showed the widest zone of inhibition and the smallest zone of inhibition. Compared the effect of different levels of concentration of Neem (Azadirachtaindica) leaves extract on the zone of inhibition of Staphylococcus aureus to the control set-up.

It was therefore hypothesized that: There is no significant difference between the levels of concentration: (a) 25%, (b) 50%, (c) 75%, (d) 100% of Neem leaves extract on Staphylococcus aureus as indicated by its zone of inhibition.

1.3   Significance of the Study

The anti-bacterial effect of Neem (Azadirachtaindica) leaves extract against Staphylococcus aureus, will be a great help in discovering a herbal medicine. More specifically, it is beneficial on the following context: Community- they can obtain a cheaper medicine using a herbal plant within their place and they can resort to the Neem Tree. Filipino people- herbal medicine can be afford by almost all of us due to its abundance in the community. Department  ofAgriculture- they will benefit on the ideas and knowledge that Neem tree can be used as an anti-bacterial medicine. Department  of Health – ideas and facts can be presented about the Neem tree that can be used as a medicine Pharmaceutical companies- a cheaper medicine will be sold and they would gain on it and they would take the idea for herbal medicine. Students- they could gain ideas about plants and can be used as a medicine and they can conduct further studies and researchers in relation to this study. Researchers- fellow researchers can get ideas and information about the uses of Neem tree especially in inhabiting bacterial growth. They can also study on the plants that is abundant in their place.

1.4 Scope and Limitations

This study was conducted to determine the anti-bacterial capacity of Neem (Azadirachtaindica) leaf extract on staphylococcus aureus in different concentrations: 25%, 50%, 75%, and 100% as indicated by its zone of inhibition. It also aimed to determine which concentrations has a significant effect. Experimental method was used in this study. The independent variables were the different levels of concentration of Neem tree leaf extract and dependent variable was the zone of inhibition exhibited on the culturedStaphylococcus aureus. This study is limited on the bacterial samples of Staphylococcus aureus. Completely Randomized Design (CRD) was used.The preparation of the extract, culture of bacteria and experimentation was conducted at West Visayas State University Biological Science Laboratory, July 2011.

1.5 Definition of Terms

Antibiotics- a remedy that was used in the study namely the Cloxacillin.

Bacterial culture – it is a process that the researchers did in culturing the bacterial sample

Cloxacillin – an antibiotic intended to treat bacterial infections was used as the positive control set-up.

Extraction – the process by which the juice is separated from other residue of the leaves. The tea extract was used to treat the Staphylococcus aureus.

Neem leaves- the part of the neem tree that was extracted and was used in the study in different levels of concertration.

Neem tree – the neem (Azadirachtaindica) tree leaf extract was used in treating Staphylococcus aureus.

Nutrient agar – was used as bacterial medium for bacterial culture.

Petri dish – a container where the nutrient agar was placed and cultured.

Phytochemical Analysis – a data in which we obtained the information about the content of the variables used such as Thylanoids, Enzymes, Alkaloids, Tannins and Saponins.

Staphylococcus aureus– the bacteria that was treated in the study.

Zone of inhibition- used term for the region inhibited by our antibacterial agent (Neem).

GET THE COMPLETE PROJECT

Not What You Are Looking For?



For Quick Help Chat with Us Now!

+234 813 292 6373

+233 541 351 187


Here's what our amazing customers are saying

Emmanuel Essential
Kogi state University
I actually took the risk,you know first time stuff But i was suprised i received as requested. I love you guys 🌟 🌟 🌟 🌟
Very Good
Azeez Abiodun
Moshood Abiola polytechnic
I actually googled and saw about iproject master, copied the number and contacted them through WhatsApp to ask for the availability of the material and to my luck they have it. So there was a delay with the project due to the covid19 pandemic. I was really scared before making the payment cause I’ve been scammed twice, they attended so well to me and that made me trust the process and made the payment and provided them with proof, I got my material in less than 10minutes
Very Good
Gbadamosi Solomon Oluwabunmi
Lasu
Swift delivery within 9 minutes of payment. Thank you project master
Excellent
JONNAH EHIS
Ajayi Crowther University, Oyo
I was scared at first when I saw your website but I decided to risk my last 3k and surprisingly I got my complete project in my email box instantly. This is so nice!!!
Excellent
MATTHEW NGBEDE
Ahmadu Bello University
I wish I knew you guys when I wrote my first degree project, it took so much time and effort then. Now, with just a click of a button, I got my complete project in less than 15 minutes. You guys are too amazing!
Excellent
Temitayo Ayodele
Obafemi Awolowo University
My friend told me about iprojectmaster website, I doubted her until I saw her download her full project instantly, I tried mine too and got it instantly, right now, am telling everyone in my school about iprojectmaster.com, no one has to suffer any more writing their project. Thank you for making life easy for me and my fellow students... Keep up the good work
Very Good
Stancy M
Abia State University, Uturu
I did not see my project topic on your website so I decided to call your customer care number, the attention I got was epic! I got help from the beginning to the end of my project in just 3 days, they even taught me how to defend my project and I got a 'B' at the end. Thank you so much iprojectmaster, infact, I owe my graduating well today to you guys...
Excellent
Ibrahim Muhammad Muhammad
Usmanu danfodiyo university, sokoto
It's a site that give researcher student's to gain access work,easier,affordable and understandable. I appreciate the iproject master teams for making my project work fast and available .I will surely,recommend this site to my friends.thanks a lot..!
Excellent
Dau Mohammed Kabiru
Kaduna State College of Education Gidan Waya
This is my first time..Your service is superb. But because I was pressed for time, I became jittery when I did not receive feedbackd. I will do more business with you and I will recommend you to my friends. Thank you.
Very Good
Joseph M. Yohanna
Thanks a lot, am really grateful and will surely tell my friends about your website.
Excellent

HOW TO GET YOUR COMPLETE PROJECT INSTANTLY

  • Select 3 Project Topics of your choice from your Department.
  • Submit the 3 topics to your Supervisor for Approval.
  • Call Our Instant Help Desk on 0813-292-6373 and Get Your Complete Project Material Instantly.
  • All project materials on this website are well researched by professionals with high level of professionalism.

FREQUENTLY ASKED QUESTIONS

How do i choose a Project Topic?

Look for your department and select any topic of your choice

I have a fresh topic that is not on your website. How do I go about it?

How fast can i get a complete project from your website?

Within 15 minutes if the exact project topic is on our website

Is it a complete research project or just materials?

It is a Complete Research Project i.e Chapters 1-5, Abstract, Table of Contents, Full References, Questionnaires / Secondary Data

One of your topics suites my project, but the case study is different. What do i do?

Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

How do i get my complete project?

Your Complete Project Material will be sent to your Email Address in Ms Word document format

Can I get my Complete Project through WhatsApp?

Yes! We can send your Complete Research Project to your WhatsApp Number

What if my Project Supervisor made some changes to a topic i picked from your website?

Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

Do you assist students with Assignment and Project Proposal?

Yes! Call Our Instant Help Desk Now: (+234) 813-292-6373 and you will be responded to immediately

What if i do not have any project topic idea at all?

Smiles! We've Got You Covered. Chat with us on WhatsApp Now to Get Instant Help: (+234) 813-292-6373

How can i trust this site?

We are well aware of fraudulent activities that have been happening on the internet. It is regrettable, but hopefully declining. However, we wish to reinstate to our esteemed clients that we are genuine and duly registered with the Corporate Affairs Commission as "PRIMEDGE TECHNOLOGY". This site runs on Secure Sockets Layer (SSL), therefore all transactions on this site are HIGHLY secure and safe!