Select Currency
Translate this page


Format: MS WORD  |  Chapter: 1-5  |  Pages: 45  |  808 Users found this project useful  |  Price NGN3,000





A major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus thuringiensis was assessed by isolating them from ecologically different soil habitats in and around Enugu metropolis. The isolate organisms were confirmed as Bacillus thuringiensis based on biochemical characterization and microscopic observation. The larvacidal activity of Bacillus thuringiensis isolates was tested against the larval of mosquito by using the standard cup bioassay. The isolates of Bacillus thuringiensis showed a significant level of variation in their larvacidal activity.




Bacillus thuringrensis (Bt) is a well known and widely studied bacterium which is known for its use in pest management. Today it is the most successful commercial xenobiotic with its worldwide application when compared with the chemical pesticides; Bacillus thuringiensis has the advantages of being biologically degradable, selectively active on pests and less likely to cause resistance. Safety of Bacillus thuringiensis formulations for humans, beneficial animals and plants explains the replacement of chemical pesticides in many countries with these environmentally friendly pest control agents. Bacillus thuringiensis was first isolated by the Japanese Scientist Ishiwata (1901) from skilkworm larvae, bombyxmori, exhibiting sotto disease. After 10 years, Berliner (1911) isolated the square gram (+) positive, spore-forming, rod shaped soil bacterium from disease flour moth larvae, Anngasta Kachmiccalla, in the Thuringia region of the Germany and named it as Bacillus thuringiensis. 

In the early 1930s Bacillus thuringiensis was used against Ostrinianubilis, the European corn borer. The first commercial product was available in 1938 in France, with the trade name sporeine (Weiser, 1986). It was Bacillus thuringiensis subspecies Kurstaki that was used for the control of the insect (Lepidopteran) pests in agriculture and forestry (Luthy & Ebersold, 1981). New commercial products arrived in 1980s after the discovering of subspecies thuringiensis opened the gate for black fly and mosquito larvae control.  Like all organisms, insect are susceptible to infection by pathogenic microorganisms, many of these infections agents have a narrow host range and therefore, do not cause uncontrolled destruction of beneficial insects and are not toxic to vertebrates. Bacillus thuringiensis is a major microorganism, which shows entamopathogenic activity (Glazer & Nikaido, 1995, Schnepf, et al. 1998) which forms parasporal crystals during the stationary phase of its growth cycle. 

Most Bacillus thuringiensis preparations available on the market contain spores with parasporal inclusion bodies composed of δ – endotoxins. In commercial production, the crystals and spores obtained from fermentation are concentrated and formulated for spray on application according to conventional Agriculture practices (Baum, Kakefuda, & Gawron-Burke, 1996). There are many strains of Bacillus thuringiensis having insecticidal activity against insect order (eg Lepidoptera, Diptera, Homoptera, Mollaphage, Coloptera). Only a few of them have been commercially developed. Bacillus thuringiensis insecticides are divided into three groups, group one has been used for the control of lepidopterans. These groups of insecticides are formulated with Bacillus thuringiensis Subspecies. Kurstaki, group two contains thesandiego and tenebrionis strains of Bacillus thuringiensisand has been applied for the control of certain celopterans and their larvae. Group three contains the Israelensis strains of Bacillus thuringiensis which has been used to control black flies and mosquitoes.


The existence of parasporal inclusions in Bt was first noted I 1915 (Berliner 1915) but their protein composition was not delineated until the 1950s (Angus 1954). Hannay (1953) detected the crystalline fine structure that is a property of most of the parasporal inclusion. Bacillus thuringiensis subspecies can synthesize more than one inclusion, which may contain different ICPs. ICPs have been called data endotoxins; however since the term endotoxin usually refers to toxin associated with the other membranes of gram-negative bacteria, comprising a core lipopoly saccharide. Depending on their ICP composition, the crystals have various forms (bipyramidal, cuboidal, flat rhomboid, or a composition with two or more crystal types. A partial correlation between crystal morphology, ICP composition, and bioactivity against target insects has been established (Bulla et al.1977). Hofte and Whitely, 1989, Lynch and Baumman, 1985).


Bacillus thuringiensis is a member of the genes Bacillus and like the other members of the taxon, has the ability to form endospores that are resistant to inactivation by heat, desiccation and organic solvent. The spore formation of the organism varies from terminal to subterminal in sporangia that are not swollen, therefore, Bacillus thuringiensis resembles other members of Bacillus species in morphology and shape (Stahly, Andrews, & Yousten, 1991). The organism is gram-positive and facultitative anaerobes. The shape of the cells of the organism is rod. The size when grown in standard liquid media varies 3 – 5um. 

The most distinguishing features of Bacillus thuringiensis from other closely related Bacillus species. (eg Bacillus anthracis, Bacillus. cereus) is the presence of the parasporal crystal body that is near to the spore outside the exosporangium during the endospore formation, which is shown in figure 1:1 (Andrews, Bibilops, & Bulla, 1985; Andrews, Faust, Wabiko, Raymond, & Bulla, 1987; Bulla, Faust, Andrews, & Goodma, 1995). Bacillus thuringiensis is an insecticide producing variant of Bacillus cereus (Gordon, Haynes, & Pang, 1973) several Bt species also produce Bacillus cereus type enterotooxin (Carlson, & Kolsto, 1993) plasmids coding for the insecticidal toxin of Bacillus thuringiensis have been transferred into B. cereus to make it a crystal producing variant of Bacillus thuringiensis(Gonzalez, Brown, Carlton, 1982) molecular methods including genomic restriction digestion analysis and 16 rRNA sequence comparison support that Bacillus thuringiensis, Bacillus anthracis and Bacillus cereus are closely relocated species and they should be considered as a single species (Carlson, Caugant, & Kolstra, 1994; Ash , Farrow, Dorsch, Stackebrandt, & Collins. 1991; Helgason et al.2000).


The classification of Bacillus thuringiensis based on the serological analysis of the flagella antigens was introduced in the early 1960s (de Barjac & Bonnefoi, 1962). This classification by serotype has been supplemented by morphological and biochemical criteria (de Barjac, 1981). Clutill (1977), explains that only 13 Bacillus thuringiensis subspecies were toxic to lepidopteran Larva only. And apparently Nematode (Narva et; al., 1991) enlarged the host range and markedly increased the number of subspecies up to the end of 1998, over 67 subspecies based on flagella H – Serovars had been identified.


Although our knowledge about Bacillus thuringiensis occurs naturally and it can also be added to an ecosystem artificially to control pest, prevalence of Bacillus thuringiensis in nature can be said as “natural” and can be isolated when there is no previous record of application of the organism for pest control. The Bacillus thuringiensis which belong to artificial habitat areas are sprayed based insecticides (mixture of spores and crystal). (Stahly et al. 1991). Thus, it is obvious that Bacillus thuringiensis is widespread in nature. However, the normal habitat of the organism is soil. The organism grows naturally as asaprophyle, feeding on dead. Organic matter, therefore, the spores of Bacillus thuringiensis persist in soil and its vegetative growth occurs when there is nutrient available. Moreover Bacillus thuringiensis has recently been isolated from marine environments (Maeda et al. 2000) and from soil of Antarctica also (Foresty & Logan 2000). 

However the true role of the bacteria is not clear. Although it produces parasporal crystal inclusions that are toxic to many orders of insects, some species of Bacillus thuringiensis from diverse environments show no insecticidal activity. The insecticidal activities of Bacillus thuringiensis are rare in nature. For example, Iriarte et al.(2000) reported that there is no relationship between mosquito breeding sites and pathogenic action level of Bacillus thuringiensis in the surveyed aquatic habitats. While another study suggested that habitat with a high density of insect were originated by the pathogenic action of this bacterium (Itoqou Apoyolo et al.1995).


At the period of the active growth cycle, the strains of Bacillus thuringiensis produce extracellular compounds; this compound might yield to virulence. These extracellular compounds include proteases, chitinases phospholipases, and vegetative conseticidal protein (Zhang et al. 1993; Sohneff et al. 1998). 
Bacillus thuringrensis also produces antibiotics compounds having antifungal activity (stab et al. 1994). However the crystal toxins are more effective then these extracellular compounds and allow the development of the bacteria in dead insect larvae. Bacillus thuringiensis strains also produce a protease, which is called inhibitor. This protein attacks and selectively destroys cecropiris and attacisis which are antibacterial proteins in insects, as a result of this, the defence response of the insect collapses. This protease activity is specific, it attacks an open hydrophobic region near C – terminus of the cecropin and it does not attack the globular proteins (Duthambar & Steiner, 1984). Other important insecticidal proteins which are unrelated to crustal proteins are vegetative insecticidal protein. These proteins are produce by some strains of Bacillus thuringiensis during vegetative growth.


Colony forms can help to distinguish Bacillus thuringiensis colonies from other Bacillus species. The organism forms white, rough colonies, which spread out and can expand over the plate very quickly. Bacillus thuringiensis strains have unswallon and ellipsoidal spores that lie in the subterminal position. The presence of parasporal crystals that are adjacent to the spore in another cell is the best criteria to distinguish Bacillus thuringiensis from other closely related Bacillus species. The size number, of parasporal inclusion and morphology may vary among Bacillus thuringiensis strains. However, four distinct crystal morphologies are apparently the typical bipyramidal crystal, related to crystal proteins (Aronson et al. 1976). Cuboidal usually associated with bipyramidal crystal (Ohba&Aizawi 1986), amorphous and composite crystals related to cry4 and cry proteins (federicet al. 1990), and flat, square crystal related to cry3 proteins (Hernstadet al. 1986, Lopezmeza & Ibarra, 1996 The classification was based in part on the possession of parasporal bodies. Bernard et al.(1997) isolated 5303 Bacillus thuringiensis from 80 different countries and 2793 of them were classified according to their crystal shape. 

Bacillus thuringiensis vary’s based on geographical or environmental location. Each habitat may contain novel Bacillus thuringiensis isolated that have more toxic effects on target insects. Intensive screening programs have been identified Bacillus thuringiensis strain from soil, plant surfaces and stored product dust samples. Therefore many strain collections have been described in the literature, such as Assian (Chak et al. 1994, Ben – Dov et al. 1997, 1999) and Maxican (Bravo et al. 1998).Therefore the aim of this study is to isolate Bacillus thuringiensis from soil sample and to isolate Bacillus thuringiensis against larva of mosquito or to determine Bacillus thuringiensis against larva of mosquito.


Not What You Are Looking For?

For Quick Help Chat with Us Now!

+234 814 010 7220

+233 541 351 187

Here's what our amazing customers are saying

Emmanuel Essential
Kogi state University
I actually took the risk,you know first time stuff But i was suprised i received as requested. I love you guys 🌟 🌟 🌟 🌟
Very Good
Azeez Abiodun
Moshood Abiola polytechnic
I actually googled and saw about iproject master, copied the number and contacted them through WhatsApp to ask for the availability of the material and to my luck they have it. So there was a delay with the project due to the covid19 pandemic. I was really scared before making the payment cause I’ve been scammed twice, they attended so well to me and that made me trust the process and made the payment and provided them with proof, I got my material in less than 10minutes
Very Good
Gbadamosi Solomon Oluwabunmi
Swift delivery within 9 minutes of payment. Thank you project master
Ajayi Crowther University, Oyo
I was scared at first when I saw your website but I decided to risk my last 3k and surprisingly I got my complete project in my email box instantly. This is so nice!!!
Ahmadu Bello University
I wish I knew you guys when I wrote my first degree project, it took so much time and effort then. Now, with just a click of a button, I got my complete project in less than 15 minutes. You guys are too amazing!
Temitayo Ayodele
Obafemi Awolowo University
My friend told me about iprojectmaster website, I doubted her until I saw her download her full project instantly, I tried mine too and got it instantly, right now, am telling everyone in my school about, no one has to suffer any more writing their project. Thank you for making life easy for me and my fellow students... Keep up the good work
Very Good
Stancy M
Abia State University, Uturu
I did not see my project topic on your website so I decided to call your customer care number, the attention I got was epic! I got help from the beginning to the end of my project in just 3 days, they even taught me how to defend my project and I got a 'B' at the end. Thank you so much iprojectmaster, infact, I owe my graduating well today to you guys...
Ibrahim Muhammad Muhammad
Usmanu danfodiyo university, sokoto
It's a site that give researcher student's to gain access work,easier,affordable and understandable. I appreciate the iproject master teams for making my project work fast and available .I will surely,recommend this site to my friends.thanks a lot..!
Dau Mohammed Kabiru
Kaduna State College of Education Gidan Waya
This is my first time..Your service is superb. But because I was pressed for time, I became jittery when I did not receive feedbackd. I will do more business with you and I will recommend you to my friends. Thank you.
Very Good
Joseph M. Yohanna
Thanks a lot, am really grateful and will surely tell my friends about your website.


  • Select 3 Project Topics of your choice from your Department.
  • Submit the 3 topics to your Supervisor for Approval.
  • Call Our Instant Help Desk on +234 814 010 7220 and Get Your Complete Project Material Instantly.
  • All project materials on this website are well researched by professionals with high level of professionalism.


How do i choose a Project Topic?

Look for your department and select any topic of your choice

I have a fresh topic that is not on your website. How do I go about it?

How fast can i get a complete project from your website?

Within 15 minutes if the exact project topic is on our website

Is it a complete research project or just materials?

It is a Complete Research Project i.e Chapters 1-5, Abstract, Table of Contents, Full References, Questionnaires / Secondary Data

One of your topics suites my project, but the case study is different. What do i do?

Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

How do i get my complete project?

Your Complete Project Material will be sent to your Email Address in Ms Word document format

Can I get my Complete Project through WhatsApp?

Yes! We can send your Complete Research Project to your WhatsApp Number

What if my Project Supervisor made some changes to a topic i picked from your website?

Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

Do you assist students with Assignment and Project Proposal?

Yes! Call Our Instant Help Desk Now: +234 814 010 7220 and you will be responded to immediately

What if i do not have any project topic idea at all?

Smiles! We've Got You Covered. Chat with us on WhatsApp Now to Get Instant Help: +234 814 010 7220

How can i trust this site?

We are well aware of fraudulent activities that have been happening on the internet. It is regrettable, but hopefully declining. However, we wish to reinstate to our esteemed clients that we are genuine and duly registered with the Corporate Affairs Commission as "PRIMEDGE TECHNOLOGY". This site runs on Secure Sockets Layer (SSL), therefore all transactions on this site are HIGHLY secure and safe!